How does it work?

GeneXplain’s Upstream Analysis is an integrated promoter – pathway analysis. It starts from any list of differentially expressed genes (DEGs), which you may have extracted from your raw data with the aid of the geneXplain platform, and comprises two main steps:
  • At first, the promoters of the differentially regulated genes are retrieved and analyzed for potential transcription factor (TF) binding sites and their combinations. From that, a set of TFs is identified that potentially have regulated the found DEGs.
  • In a second step, the pathways are reconstructed that are known to activate the previously hypothesized TFs. Molecules where these pathways converge are considered as potential master regulators of the process under study