Unlock the Power of Free Gene-regulation.com

Ready to explore the fundamental drivers of gene expression?

In this webinar, we will show you how to launch your research journey at gene-regulation.com using the free access to TRANSFAC®, the renowned database for transcription factors and their binding sites.

Discover how to tap into rich regulatory information and set the foundation for groundbreaking insights in genomics, transcriptomics, and systems biology.

Start Your Gene Regulation Journey

Date: 13th May Time: 5PM CET

What You Will Learn

  • How to access and navigate Gene-regulation.com
  • Key features of the free TRANSFAC® database
  • How to identify transcription factor binding sites (TFBS) and regulated genes
  • Practical workflows to start your gene regulatory analysis
  • How to transition from free to advanced tools for deeper analysis

Why You Should Attend

gene-regulation.com provides free access to essential gene regulation data that can accelerate your research.

Understanding how genes are regulated is fundamental to advancing fields like cancer biology and developmental studies.

In this webinar, you will learn how to leverage powerful tools such as Match, P-Match, and CMA within the free TRANSFAC® platform to identify transcription factor binding sites regulating genes of interest. 

And if you’re already thinking, “What’s next?” — we’ve got you covered. Get an exclusive look at how you can advance your research even further by uncovering master regulators and drug targets with the full professional power of TRANSFAC®.

Who Should Attend

  • Researchers and students in genomics, transcriptomics, bioinformatics, and molecular biology
  • Scientists starting projects involving transcription factors or gene regulatory networks
  • Bioinformaticians exploring new resources for data-driven research

What to expect – and what not to expect – from TRANSFAC Free (Public)

Compared to Pro version

TRANSFAC free (public)

TRANSFAC professional

Data

Factors

6,133

49,372

miRNAs

n/a

1,772

DNA sites

7,195

50,891

mRNA sites

n/a

67,700

Factor-DNA site links

Yes

68,898

miRNA-mRNA site links

n/a

74,550

Genes

2,397

103,765

ChIP-Seq fragments

n/a

112,344,359

Matrices

398

11,124

References

Yes

45,665

Promoter sequences

n/a

465,413

Enhancer / silencer sequences

n/a

231,413

Search Options

Use of Boolean operators / advanced filtering

No

Yes

Ontology search

No

Yes

Customizable / exportable search results

No

Yes

Export in FASTA and BED format

No

Yes

Analysis Capabilities

MATCH Suite: toolbox to analyze syntax and semantics of gene regulation

No

Yes

Analysis workflows for ChIP-Seq and RNA-Seq data

No

Yes

Identify shared functional attributes

No

Yes

Data Download

Flat file and JSON format data files

No

Yes

Free TRANSFAC public (gene-regulation.com):
Solving Real Research Pains

Task 1

I cloned a promoter but don’t know which TFs might regulate it

Tool / Section

MATCH™

How Free TRANSFAC Helps

Predict TFBS motifs in your sequence

How Professional TRANSFAC Helps

Access to full PWM library (>10,000 matrices), covering all major TF families and conditions → higher accuracy and fewer false negatives

Task 2

I want to predict TFs enriched in a DEG list from RNA-seq

Tool / Section

F-MATCH™, CMA (Composite Module Analyst)

How Free TRANSFAC Helps

Analyze shared TF motifs across promoters using F-MATCH and CMA

How Professional TRANSFAC Helps

Perform true enrichment analysis with statistical scoring and promoter library customization for any organism

Task 3

I’m teaching TFBS analysis to students

Tool / Section

MATCH™ + Matrix Browser

How Free TRANSFAC Helps

Simple demo of TF motif scanning

How Professional TRANSFAC Helps

Use advanced tools like Upstream Analysis, network reconstruction, and tissue-specific promoter analysis in teaching and research

Task 4

I want to compare TFBS profiles between species or promoter variants

Tool / Section

MATCH™

How Free TRANSFAC Helps

Manually scan sequences one by one

How Professional TRANSFAC Helps

Can perform batch analysis, cross-species promoter comparisons

Task 5

I want to mutate key TF binding sites in my promoter construct

Tool / Section

MATCH™

How Free TRANSFAC Helps

Find predicted motif locations

How Professional TRANSFAC Helps

Prioritize biologically relevant sites using ChIP-seq support and binding affinity scores from ML-based approach

Task 6

I have a candidate TF and want to explore its binding behavior

Tool / Section

Matrix Browser

How Free TRANSFAC Helps

View matrix information and consensus sequence

How Professional TRANSFAC Helps

Access experimentally supported matrices with detailed binding context (e.g., cell type, condition, tissue)

Task 7

I want to match motifs using flexible scoring and conservation

Tool / Section

P-Match

How Free TRANSFAC Helps

Use P-Match for combined scoring

How Professional TRANSFAC Helps

Tune search with custom thresholds, apply to entire gene sets, and integrate into graphical workflows

Task 8

I need to detect TFs in a highly variable or unknown promoter

Tool / Section

MATCH™, AliBaba2

How Free TRANSFAC Helps

AliBaba2 allows fuzzy motif matching

How Professional TRANSFAC Helps

Use modern, AI-assisted scanning with context-aware scoring, multi-matrix matching, and support for enhancers

Task 9

I want to analyze 100–1000 genes from my omics study

Tool / Section

MATCH™ + manual input

How Free TRANSFAC Helps

Manual MATCH™ input one-by-one

How Professional TRANSFAC Helps

Fully integrated into graphical workflows for multiple input

Task 10

I want to find master-regulators driving expression in my omics data

How Free TRANSFAC Helps

Not supported in free version

How Professional TRANSFAC Helps

Run Upstream Analysis: connects TFBS to signaling cascades and identifies key master regulators with biological causality

Usage of TRANSFAC

Best for:

  • Students, educators, and early-stage researchers
  • Single-sequence TFBS scanning
  • Initial hypothesis generation or construct design

Not ideal for:

  • Omics-based TF prioritization
  • Tissue- or disease-specific regulation
  • Master regulator inference or pathway analysis
  • High-throughput or genome-wide workflows